Publications
Ugur E, de la Porte A, Qin W, Bultmann S, Ivanova A, Drukker M, Mann M, Wierer M and Leonhardt H (2023). Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components. Nucleic Acids Res., 51, 2671-2690. PubMed
Miyashita R, Nishiyama A, Qin W, Chiba Y, Kori S, Kato N, Konishi C, Kumamoto S, Kozuka-Hata H, Oyama M, Kawasoe Y, Tsurimoto T, Takahashi TS, Leonhardt H, Arita K and Nakanishi M (2023). The termination of UHRF1-dependent PAF15 ubiquitin signaling is regulated by USP7 and ATAD5. Elife, 12, e79013. PubMed
Zhang H, Qin W, Romero H, Leonhardt H and Cardoso MC (2023). Heterochromatin organization and phase separation. Nucleus, 14, 2159142. PubMed
Hata K, Kobayashi N, Sugimura K, Qin W, Haxholli D, Chiba Y, Yoshimi S, Hayashi G, Onoda H, Ikegami T, Mulholland CB, Nishiyama A, Nakanishi M, Leonhardt H, Konuma T and Arita K (2022). Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger. Nucleic Acids Res., 50, 12527-12542. PubMed
Qin W, Steinek C, Kolobynina K, Forné I, Imhof A, Cardoso MC and Leonhardt H (2022). Probing protein ubiquitination in live cells. Nucleic Acids Res., 50, e125. PubMed
Zhang H, Romero H, Schmidt A, Gagova K, Qin W, Bertulat B, Lehmkuhl A, Milden M, Eck M, Meckel T, Leonhardt H and Cardoso MC (2022). MeCP2-induced heterochromatin organization is driven by oligomerization-based liquid–liquid phase separation and restricted by DNA methylation. Nucleus, 13, 1-34. PubMed
Qin W, Ugur E, Mulholland CB, Bultmann S, Solovei I, Modic M, Smets M, Wierer M, Forné I, Imhof A, Cardoso MC and Leonhardt H (2021). Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency. Nucleic Acids Res., 49, 7406-7423. PubMed
Qin W, Stengl A, Ugur E, Leidescher S, Ryan J, Cardoso MC and Leonhardt H (2021). HP1β carries an acidic linker domain and requires H3K9me3 for phase separation. Nucleus, 12, 44-57. PubMed
Mulholland CB, Nishiyama A, Ryan J, Nakamura R, Yiğit M, Glück IM, Trummer C, Qin W, Bartoschek MD, Traube FR, Parsa E, Ugur E, Modic M, Acharya A, Stolz P, Ziegenhain C, Wierer M, Enard W, Carell T, Lamb DC, Takeda H, Nakanishi M, Bultmann S and Leonhardt H (2020). Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals. Nat Commun., 11, 5972. PubMed
Nishiyama A, Mulholland CB, Bultmann S, Kori S, Endo A, Saeki Y, Qin W, Trummer C, Chiba Y, Yokoyama H, Kumamoto S, Kawakami T, Hojo H, Nagae G, Aburatani H, Tanaka K, Arita K, Leonhardt H and Nakanishi M (2020). Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation. Nat Commun., 11, 1222. PubMed
Karg E, Smets M, Ryan J, Forné I, Qin W, Mulholland CB, Kalideris G, Imhof A, Bultmann S and Leonhardt H (2017). Ubiquitome analysis reveals PCNA-associated factor 15 (PAF15) as a specific ubiquitination target of UHRF1 in embryonic stem cells. J Mol Biol., 429, 3814-3824. PubMed
Gressel S, Schwalb B, Decker TM, Qin W, Leonhardt H, Eick D and Cramer P (2017). CDK9-dependent RNA polymerase II pausing controls transcription initiation. eLife, 6, e29736. PubMed
Smets M, Link S, Wolf P, Schneider K, Solis V, Ryan J, Meilinger D, Qin W and Leonhardt H (2017). DNMT1 mutations found in HSANIE patients affect interaction with UHRF1 and neuronal differentiation. Hum. Mol. Genet., 26, 1522-1534. PubMed
Qin W, Wolf P, Liu N, Link S, Smets M, Mastra F, Forné I, Pichler G, Hörl D, Fellinger K, Spada F, Bonapace IM, Imhof A, Harz H and Leonhardt H (2015). DNA methylation requires a DNMT1 ubiquitin interacting motif (UIM) and histone ubiquitination. Cell Res., 25, 911-29. PubMed
Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H and Bultmann S (2015). A modular open platform for systematic functional studies under physiological conditions. Nucleic Acids Res., 43, e112. PubMed
Liu N, Wang M, Deng W, Schmidt CS, Qin W, Leonhardt H and Spada F (2013). Intrinsic and Extrinsic Connections of Tet3 Dioxygenase with CXXC Zinc Finger Modules. PLoS One, 8, e62755. PubMed
Schneider K, Fuchs C, Dobay A, Rottach A, Qin W, Wolf P, Álvarez-Castro J, Nalaskowski M, Kremmer E, Schmid V, Leonhardt H and Schermelleh L (2013). Dissection of cell cycle-dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modeling. Nucleic Acids Res., 41, 4860-4876. PDF, PubMed
Qin W, Leonhardt H and Pichler G (2011). Regulation of DNA methyltransferase 1 by interactions and modifications. Nucleus, 2, 392-402. PubMed
Frauer C, Rottach A, Meilinger D, Bultmann S, Fellinger K, Hasenöder S, Wang M, Qin W, Söding J, Spada F and Leonhardt H (2011). Different Binding Properties and Function of CXXC Zinc Finger Domains in Dnmt1 and Tet1. PLoS ONE 6(2): e16627. PubMed
Qin W, Leonhardt H and Spada F (2011). Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1. J. Cell Biochem. 112, 439–444. PubMed.